Rapid fluorescence tagging of glycans and other biomolecules with enhanced MS signals

ABSTRACT

Mass spectrometry (MS) active, fluorescent rapid tagging reagent is provided having three substituent groups: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety; and (c) a reactive group that can react with an amine. The reactive group provides rapid tagging of desired bio-molecules. The fluorescent moiety provides the fluorescent signal. The tertiary amino group provides the MS signal.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of U.S. patent application Ser. No. 14/193,418, which is a continuation of International Application No. PCT/US2012/057996, filed Feb. 28, 2014, which claims the benefit of Provisional Application No. 61/540,306, filed Sep. 28, 2011, and also a continuation-in-part of U.S. patent application Ser. No. 14/342,131, which is the National Stage of International Application No. PCT/US2012/057996, filed Feb. 28, 2014, which claims the benefit of Provisional Application No. 61/540,306.

STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

None.

THE NAMES OF PARTIES TO A JOINT RESEARCH AGREEMENT

None.

BACKGROUND OF THE INVENTION

Fluorescent labeling of N-glycans is beneficial to detecting glycans because it improves both sensitivity and selectivity of the detection as well as the chromatographic behavior of glycans. Amino acid analysis is a fundamental process in protein research and is of particular importance to clinical chemists and pharmaceutical manufacturers when glycosylation profiling of proteins must be monitored to ensure consistency of a therapeutic product. Upon derivation with a reagent having a fluorescent moiety, the functional group of the compound can only be estimated. Mass spectrometry (“MS”) is then required to identify the specific compound.

Analysis by MS has reached a high degree of development with respect to glycan and amino acid analysis and proteomics. However, the necessary reaction time to functionalize most amines is slow. The current state of the art utilizes tagging molecules that either (1) react quickly and have good fluorescence signal, but poor MS signal or (2) react very slowly and give good MS/fluorescence signals. A combination of MS and fluorescence detection is desirable, however, because fluorescence detection is very useful tool in determining quantitatively how much is present. On the other hand, MS is used to determine what the molecular makeup is.

A need exists, therefore, for molecules with that rapidly react with biomolecules and provide strong mass spectrometry and fluorescence signals.

SUMMARY OF THE INVENTION

Described herein are MS active compounds useful in rapid fluorescence tagging of glycans such as N-linked glycans and other bio-molecules including, but not limited to, proteins, peptides and amino acids. These MS active, fluorescent compounds have three functional components: (a) a tertiary amino group or other MS active atom; (b) a highly fluorescent moiety, and (c) a functional group that rapidly reacts with amines, such as an isocynanate or succidimidylcarbamate. The reactive functional group provides rapid tagging of desired bio-molecules, and the fluorescent moiety provides for a strong fluorescent signal. The tertiary amino group substituent provides a strong MS signal.

In particular, the invention relates to compounds of the various formulas described herein. Each compound can act as a reagent for rapid fluorescence tagging of biomolecules and enhanced MS signaling. For example, the MS active, rapid fluorescence tagging compounds can be of the structural Formula IV:

-   -   or a salt or solvate thereof, wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

Furthermore, the MS active, rapid fluorescence tagging compounds can be of the structural Formula V:

-   -   or a salt or solvate thereof, wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is independently selected from O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N.

The compounds of the formulas described herein may have optical centers and therefore may occur in different enantiomeric and disastereomeric configurations. The invention described herein includes all enantiomers, diastereomers and other stereoisomers of such compounds of each formula, as well as racemic compounds and racemic mixtures and other mixtures of stereoisomers thereof.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows the fluorescence and MS detection of the glycans G0F, G1F, and G2F released from 0.8 μg Herceptin IgG labeled with 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate.

FIG. 2 shows the fluorescence and MS detection of the glycan G2FBS2 released from 0.8 μg Herceptin IgG labeled with 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate.

DETAILED DESCRIPTION

Novel compounds useful in the rapid fluorescence tagging of glycans such as N-linked glycans and other bio-molecules including, but not limited to, proteins, peptides and amino acids and with enhanced MS signaling are provided herein. These compounds are used to analyze glycans and/or other biomolecules in a sample. To analyze the biomolecule, the molecule is rapidly labeled with a compound described herein and then subjected to liquid chromatography, mass spectrometry, and fluorescence detection.

The term “alkoxy,” as used herein, alone or in combination, refers to an alkyl ether radical, wherein the term alkyl is as defined below. Examples of suitable alkyl ether radicals include methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, iso-butoxy, sec-butoxy, tert-butoxy, and the like.

The term “alkyl,” as used herein, alone or in combination, refers to a straight-chain or branched-chain alkyl radical containing from 1 to and including 20, preferably 1 to 10, and more preferably 1 to 6, carbon atoms. Alkyl groups may be optionally substituted as defined herein. Examples of alkyl radicals include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, pentyl, iso-amyl, hexyl, octyl, noyl and the like. The term “alkylene,” as used herein, alone or in combination, refers to a saturated aliphatic group derived from a straight or branched chain saturated hydrocarbon attached at two or more positions, such as methylene (—CH₂—).

The term “alkylamino,” as used herein, alone or in combination, refers to an alkyl group attached to the parent molecular moiety through an amino group. Suitable alkylamino groups may be mono- or dialkylated, forming groups such as, for example, N-methylamino, N-ethylamino, N,N-dimethylamino, N,N-ethylmethylamino and the like.

The term “amino,” as used herein, alone or in combination, refers to —NRR′, wherein R and R′ are independently selected from the group consisting of hydrogen, alkyl, acyl, heteroalkyl, aryl, cycloalkyl, heteroaryl, and heterocycloalkyl, any of which may themselves be optionally substituted.

The term “aryl,” as used herein, alone or in combination, means a carbocyclic aromatic system containing one, two or three rings wherein such rings may be attached together in a pendent manner or may be fused. The term “aryl” embraces aromatic radicals such as benzyl, phenyl, naphthyl, anthracenyl, phenanthryl, indanyl, indenyl, annulenyl, azulenyl, tetrahydronaphthyl, and biphenyl.

The terms “benzo” and “benz,” as used herein, alone or in combination, refer to the divalent radical C₆H₄═ derived from benzene. Examples include benzothiophene and benzimidazole.

The term “carbamate,” as used herein, alone or in combination, refers to an ester of carbamic acid (—NHCOO—) which may be attached to the parent molecular moiety from either the nitrogen or acid end, and which may be optionally substituted as defined herein.

The term “O-carbamyl” as used herein, alone or in combination, refers to a —OC(O)NRR′, group-with R and R′ as defined herein.

The term “N-carbamyl” as used herein, alone or in combination, refers to a ROC(O)NR′— group, with R and R′ as defined herein.

The term “carbonyl,” as used herein, when alone includes formyl [—C(O)H] and in combination is a —C(O)— group.

The term “carboxy,” as used herein, refers to —C(O)OH or the corresponding “carboxylate” anion, such as is in a carboxylic acid salt. An “O-carboxy” group refers to a RC(O)O— group, where R is as defined herein. A “C-carboxy” group refers to a —C(O)OR groups where R is as defined herein.

The term “cycloalkyl” refers to a carbocyclic substituent obtained by removing a hydrogen from a saturated carbocyclic molecule and having three to fourteen carbon atoms. In one embodiment, a cycloalkyl substituent has three to ten carbon atoms. Examples of cycloalkyl include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

The term “halo,” or “halogen,” as used herein, alone or in combination, refers to fluorine, chlorine, bromine, or iodine.

The term “haloalkoxy,” as used herein, alone or in combination, refers to a haloalkyl group attached to the parent molecular moiety through an oxygen atom.

The term “haloalkyl,” as used herein, alone or in combination, refers to an alkyl radical having the meaning as defined above wherein one or more hydrogens are replaced with a halogen. Specifically embraced are monohaloalkyl, dihaloalkyl and polyhaloalkyl radicals. A monohaloalkyl radical, for one example, may have an iodo, bromo, chloro or fluoro atom within the radical. Dihalo and polyhaloalkyl radicals may have two or more of the same halo atoms or a combination of different halo radicals. Examples of haloalkyl radicals include fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, dichloromethyl, trichloromethyl, pentafluoroethyl, heptafluoropropyl, difluorochloromethyl, dichlorofluoromethyl, difluoroethyl, difluoropropyl, dichloroethyl and dichloropropyl. “Haloalkylene” refers to a haloalkyl group attached at two or more positions. Examples include fluoromethylene (—CFH—), difluoromethylene (—CF₂—), chloromethylene (—CHCl—) and the like.

The term “heteroalkyl,” as used herein, alone or in combination, refers to a stable straight or branched chain, or cyclic hydrocarbon radical, or combinations thereof, fully saturated or containing from 1 to 3 degrees of unsaturation, consisting of the stated number of carbon atoms and from one to three heteroatoms selected from the group consisting of O, N, and S, and wherein the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen heteroatom may optionally be quaternized. The heteroatom(s) O, N and S may be placed at any interior position of the heteroalkyl group. Up to two heteroatoms may be consecutive, such as, for example, —CH₂—NH—OCH₃.

The terms “heterocycloalkyl” and, interchangeably, “heterocycle,” as used herein, alone or in combination, each refer to a saturated, partially unsaturated, or fully unsaturated monocyclic, bicyclic, or tricyclic heterocyclic radical containing at least one, preferably 1 to 4, and more preferably 1 to 2 heteroatoms as ring members, wherein each said heteroatom may be independently selected from the group consisting of nitrogen, oxygen, and sulfur, and wherein there are preferably 3 to 8 ring members in each ring, more preferably 3 to 7 ring members in each ring, and most preferably 5 to 6 ring members in each ring. “Heterocycloalkyl” and “heterocycle” are intended to include sulfones, sulfoxides, N-oxides of tertiary nitrogen ring members, and carbocyclic fused and benzo fused ring systems; additionally, both terms also include systems where a heterocycle ring is fused to an aryl group, as defined herein, or an additional heterocycle group. Heterocycle groups of the invention are exemplified by aziridinyl, azetidinyl, 1,3-benzodioxolyl, dihydroisoindolyl, dihydroisoquinolinyl, dihydrocinnolinyl, dihydrobenzodioxinyl, dihydro[1,3]oxazolo[4,5-b]pyridinyl, benzothiazolyl, dihydroindolyl, dihy-dropyridinyl, 1,3-dioxanyl, 1,4-dioxanyl, 1,3-dioxolanyl, isoindolinyl, morpholinyl, piperazinyl, pyrrolidinyl, tetrahydropyridinyl, piperidinyl, thiomorpholinyl, and the like. The heterocycle groups may be optionally substituted unless specifically prohibited.

The term “optionally substituted” means the anteceding group may be substituted or unsubstituted. When substituted, the substituents of an “optionally substituted” group may include, without limitation, one or more substituents independently selected from the following groups or a particular designated set of groups, alone or in combination: lower alkyl, lower alkenyl, lower alkynyl, lower alkanoyl, lower heteroalkyl, lower heterocycloalkyl, lower haloalkyl, lower haloalkenyl, lower haloalkynyl, lower perhaloalkyl, lower perhaloalkoxy, lower cycloalkyl, phenyl, aryl, aryloxy, lower alkoxy, lower haloalkoxy, oxo, lower acyloxy, carbonyl, carboxyl, lower alkylcarbonyl, lower carboxyester, lower carboxamido, cyano, hydrogen, halogen, hydroxy, amino, lower alkylamino, arylamino, amido, nitro, thiol, lower alkylthio, arylthio, lower alkylsulfinyl, lower alkylsulfonyl, arylsulfinyl, arylsulfonyl, arylthio, sulfonate, sulfonic acid, trisubstituted silyl, N₃, SH, SCH₃, C(O)CH₃, CO₂CH₃, CO₂H, pyridinyl, thiophene, furanyl, lower carbamate, and lower urea. Two substituents may be joined together to form a fused five-, six-, or seven-membered carbocyclic or heterocyclic ring consisting of zero to three heteroatoms, for example forming methylenedioxy or ethylenedioxy. An optionally substituted group may be unsubstituted (e.g., —CH₂CH₃), fully substituted (e.g., —CF₂CF₃), monosubstituted (e.g., —CH₂CH₂F) or substituted at a level anywhere in-between fully substituted and monosubstituted (e.g., —CH₂CF₃). Where substituents are recited without qualification as to substitution, both substituted and unsubstituted forms are encompassed. Where a substituent is qualified as “substituted,” the substituted form is specifically intended. Additionally, different sets of optional substituents to a particular moiety may be defined as needed; in these cases, the optional substitution will be as defined, often immediately following the phrase, “optionally substituted with.”

The term R or the term R′, appearing by itself and without a number designation, unless otherwise defined, refers to a moiety selected from the group consisting of hydrogen, alkyl, cycloalkyl, heteroalkyl, aryl, heteroaryl and heterocycloalkyl, any of which may be optionally substituted. Such R and R′ groups should be understood to be optionally substituted as defined herein. Whether an R group has a number designation or not, every R group, including R, R′ and R^(n) where n=(1, 2, 3, . . . n), every substituent, and every term should be understood to be independent of every other in terms of selection from a group. Should any variable, substituent, or term (e.g. aryl, heterocycle, R, etc.) occur more than one time in a formula or generic structure, its definition at each occurrence is independent of the definition at every other occurrence. Those of skill in the art will further recognize that certain groups may be attached to a parent molecule or may occupy a position in a chain of elements from either end as written. Thus, by way of example only, an unsymmetrical group such as —C(O)N(R)— may be attached to the parent moiety at either the carbon or the nitrogen.

The term “bond” refers to a covalent linkage between two atoms, or two moieties when the atoms joined by the bond are considered to be part of larger substructure. A bond may be single, double, or triple unless otherwise specified. A dashed line between two atoms in a drawing of a molecule indicates that an additional bond may be present or absent at that position. The development and production of therapeutic proteins is becoming the fastest-growing segment of the pharmaceutical industry. The efficacy, stability and protein secretion of these large molecule drugs depend on their Post Translational Modifications (“PTMs”). Glycosylation is the most complex and common PTM and plays a vital role in the safety and efficacy of many therapeutic proteins such as recombinant antibodies. Several studies have shown the correlation between glycosylation variations caused by cell line selection and changes in culture medium parameters. Patrick Hossler et al., Optimal and Consistent Protein Glycosylation in Mammalian Cell Culture, 19 GLYCOBIOLOGY 926 (2009). These variations can have a profound effect on the biological activities of the mAb drugs, which leads to changes in drug potency in the final product. Regulatory agencies require monitoring of batch-to-batch recombinant antibody drug production quality and mandate detailed assessment of the protein glycosylation micro-heterogeneity and consistency.

The compounds described herein may also form hydrogen bonds with other compounds. A hydrogen bond is an electromagnetic attractive interaction between polar molecules, where hydrogen is bonded to an electronegative atom such as nitrogen or oxygen. The hydrogen bond represents a strong dipole-dipole attraction. These hydrogen-bond attractions can occur between molecules (intermolecular) or within different parts of a single molecule (intramolecular). When a hydrogen atom is attached to an electronegative atom, it is considered a hydrogen bond donor. The electronegative atom is considered a hydrogen bond acceptor, whether it is bonded to a hydrogen atom or not. While many possible hydrogen bonds can exist between two compounds, an exemplary bonding of a compound with another could take one of many forms, including, but not limited to, for example the following:

A general representation for hydrogen bonded compounds is provided immediately below:

where the dot represents a first compound hydrogen bonded to a second compound, but not the exact bonding between such compounds, and hydrogen bonds could be formed at different positions within compounds.

Asymmetric centers exist in the compounds presented herein. These centers are designated by the symbols “R” or “S,” depending on the configuration of substituents around the chiral carbon atom. It should be understood that the invention encompasses all stereochemical isomeric forms, including diastereomeric, enantiomeric, and epimeric forms, as well as d-isomers and l-isomers, and mixtures thereof. Individual stereoisomers of compounds can be prepared synthetically from commercially available starting materials which contain chiral centers or by preparation of mixtures of enantiomeric products followed by separation such as conversion to a mixture of diastereomers followed by separation or recrystallization, chromatographic techniques, direct separation of enantiomers on chiral chromatographic columns, or any other appropriate method known in the art. Starting compounds of particular stereochemistry are either commercially available or can be made and resolved by techniques known in the art. Additionally, the compounds of the present invention may exist as geometric isomers. The present invention includes all cis, trans, syn, anti, entgegen (E), and zusammen (Z) isomers as well as the appropriate mixtures thereof. Additionally, compounds may exist as tautomers; all tautomeric isomers are provided by this invention. Additionally, the compounds of the present invention can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like. In general, the solvated forms are considered equivalent to the unsolvated forms for the purposes of the present invention.

Hence, the compounds described herein can also be in the form of a salt or solvate, in particular acid addition salts. Through a reaction with either organic or inorganic acids, compounds presented herein or groups of compounds can form a salt. For example, in acid-base neutralization, an acid and a base react to form water and a salt. Basically, to react together, there must be the transfer of protons between acids and bases. Also, different acids can produce different ions. For example, an Arrhenius acid produces hydronium ions when it dissociates in water. Similarly, a Bronsted-Lowry acid is a proton donor that donates hydrogen ions to the base. Hence, proton acceptors and proton donors are the basis for the reaction and are referred to sometimes as a conjugate base or a conjugate acid. A conjugate pair refers to acids and bases with common features, where there is an equal loss/gain of protons between the pairs. For example NH₄ ⁺ is the conjugate acid to the base NH₃ because NH₃ gains a hydrogen ion to form NH₄ ⁺ as H₂O donates a hydrogen ion to form OH⁻, the conjugate base. On the other hand, under a different theory, a Lewis acid accepts an electron pair and a Lewis base donates an electron pair donor. Accordingly, the proton H⁺ can be an electron pair acceptor. Moreover, a compound can be both, a Lewis acid and a Lewis base, depending on the reaction. For example, methyl iodide can behave as both, a Lewis acid and a Lewis base, where the methyl group is donated to form a salt.

Examples of acids which can be employed to form a salt of any of the compounds provided herein include inorganic acids and organic acids as well known to those skilled in the art such as, but not limited to, N-hydroxysuccinimide, hydrochloric, hydrofluoric, hydroiodic, hydrobromic, sulfuric, hydrosulfuric, thiosulfuric, hydrocyanic, phosphoric, phosphorous, hydrochlorous, chlorous, nitrous, nitric, chloric, perchloric, sulfurous, oxalic, maleic, succinic, and citric. Salts may also be formed by coordination of the compounds with an alkali metal or alkaline earth ion. In addition, other acids can form a salt including, but not limited to, 1-hydroxy-2-naphthoic acid, 2,2-dichloroacetic acid, 2-hydroxyethanesulfonic acid, 2-oxoglutaric acid, 4-acetamidobenzoic acid, 4-aminosalicylic acid, acetic acid, adipic acid, ascorbic acid (L), aspartic acid (L), benzenesulfonic acid, benzoic acid, camphoric acid (+), camphor-10-sulfonic a cid (+), capric acid (decanoic acid), caproic acid (hexanoic acid), caprylic acid (octanoic acid), carbonic acid, cinnamic acid, citric acid, cyclamic acid, dodecylsulfuric acid, ethane-1,2-disulfonic acid, ethanesulfonic acid, ethanesulfonic acid, formic acid, fumaric acid, galactaric acid, gentisic acid, glucoheptonic acid (D), gluconic acid (D), glucuronic acid (D), glutamic acid, glutaric acid, glycerophosphoric acid, isobutyric acid, lactic acid (DL), lactobionic acid, lauric acid, maleic acid, malic acid (−L), malonic acid, mandelic acid (DL), methanesulfonic acid, naphthalene-1,5, disulfonic acid, naphthalene-2-sulfonic acid, nicotinic acid, nitric acid, oleic acid, oxalic acid, palmitic acid, pamoic acid, phosphoric acid, proprionic acid, pyroglutamic acid (−L), salicylic acid, sebacic acid, stearic acid, succinic acid, sulfuric acid, tartaric acid (+L), thiocyanic acid, toluenesulfonic acid (p), undecylenic acid.

For the compounds described herein, the counterion can be the conjugate base formed after reacting a compound or groups of compounds with an acid. In other words, counterion holds the opposite charge to that of the compound or compounds it is associated with. Thus, with respect to possible salts of the compounds herein having a conjugate acid of NH₄ ⁺, the counterion represents the anionic part of the salt.

Hence, counterions of a salt compound described herein can include, but are not limited to, any of the following common anions and oxoanions: N-hydroxysuccinimidyl, hydride (H⁻), fluoride (F⁻), chloride (C⁻), bromide (Br⁻), iodide (I⁻), oxide (O²⁻), hydroxide (OH⁻), peroxide (O₂ ²⁻), sulfide (S²⁻), hydrogen sulfide (HS⁻), selenide (Se²⁻), nitride (N³⁻), azide (N₃ ⁻), phosphide (P³⁻), arsinide (As³⁻), carbide (C⁴⁻), cyanide (CN⁻), hypochlorite (ClO₁ ⁻), chlorite (ClO₂ ⁻), chlorate (ClO₃ ⁻), perchlorate (ClO₄ ⁻), sulfite (SO₃ ²⁻), sulfate (SO₄ ²⁻), hydrogen sulfate (HSO₄ ⁻), thiosulfate (S₂O₃ ²⁻), nitrite (NO₂ ⁻), nitrate (N₃ ⁻), phosphite (PO₃ ²⁻), phosphate (PO₄ ³⁻), (mono)hydrogen phosphate (HPO₄ ²⁻), dihydrogen phosphate (H2PO₄ ⁻), carbonate (CO₃ ²⁻), hydrogen carbonate (HCO₃ ⁻), oxalate (C₂O₄ ²⁻), cyanate (NCO⁻), isocyanate (OCN⁻), thiocyanate (SCN⁻), chromate (CrO₄ ²⁻), dichromate (Cr₂O₇ ²⁻), permanganate (MnO₄ ⁻).

For example, provided herein are salt forms of compounds of the structural Formula I:

-   -   wherein     -   R¹ is O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or —C═N—;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—;     -   R⁷ is independently selected from —H or methyl; and     -   Y is a counterion.

Similarly, compounds of the structural Formula II in a salt form are:

-   -   wherein     -   m=0-9;     -   n=0-9;     -   R¹ is independently selected from O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring;     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N;     -   R⁷ is independently selected from —H or methyl; and     -   Y is a counterion.

Likewise, compounds of the structural Formula III are provided and shown in a salt form:

-   -   wherein     -   x=0 or 1;     -   m=0-9;     -   n=0-9;     -   Ar is selected from optionally substituted phenyl group,         naphthyl group, anthryl group, pyridyl group, pyrazyl group,         quinolyl group, acridly group, and coumaryl group, and in         combination with a carbonyl group produces a fluorescent moiety;     -   R² is a reactive group selected from the group consisting of         N-succinimidyl carbamate, isocyanate, and isothiocyanate;     -   R³ and R⁴ are independently selected from the group consisting         of —H, alkyl, alkyl amino, alkylsulfonic acid, and alkyl         phosphonic acid, lower alkyl, lower alkenyl, lower alkynyl,         lower alkanoyl, lower heteroalkyl, lower heterocycloalkyl, lower         haloalkyl, lower haloalkenyl, lower haloalkynyl, lower         perhaloalkyl, lower perhaloalkoxy, lower cycloalkyl, phenyl,         aryl, aryloxy, lower alkoxy, lower haloalkoxy, oxo, lower         acyloxy, carbonyl, carboxyl, lower alkylcarbonyl, lower         carboxyester, lower carboxamido, cyano, hydrogen, halogen,         hydroxy, amino, lower alkylamino, arylamino, amido, nitro,         thiol, lower alkylthio, arylthio, lower alkylsulfinyl, lower         alkylsulfonyl, arylsulfinyl, arylsulfonyl, arylthio, sulfonate,         sulfonic acid, trisubstituted silyl, N₃, SH, SCH₃, C(O)CH₃,         CO₂CH₃, CO₂H, pyridinyl, thiophene, furanyl, lower carbamate,         and lower urea, wherein two substituents can be joined together         to form a fused five-, six-, or seven-membered carbocyclic or         heterocyclic ring consisting of zero to three heteroatoms and         the optionally substituted group can be unsubstituted, fully         substituted, monosubstituted or substituted at a level anywhere         in-between fully substituted and monosubstituted and R³ and R⁴,         alone or in combination, can be MS active;     -   R⁵ is lower alkyl, lower alkenyl, lower alkynyl, lower alkanoyl,         lower heteroalkyl, lower heterocycloalkyl, lower haloalkyl,         lower haloalkenyl, lower haloalkynyl, lower perhaloalkyl, lower         perhaloalkoxy, lower cycloalkyl, phenyl, aryl, aryloxy, lower         alkoxy, lower haloalkoxy, oxo, lower acyloxy, carbonyl,         carboxyl, lower alkylcarbonyl, lower carboxyester, lower         carboxamido, cyano, hydrogen, halogen, hydroxy, amino, lower         alkylamino, arylamino, amido, nitro, thiol, lower alkylthio,         arylthio, lower alkylsulfinyl, lower alkylsulfonyl,         arylsulfinyl, arylsulfonyl, arylthio, sulfonate, sulfonic acid,         trisubstituted silyl, N₃, SH, SCH₃, C(O)CH₃, CO₂CH₃, CO₂H,         pyridinyl, thiophene, furanyl, lower carbamate, and lower urea,         wherein two substituents can be joined together to form a fused         five-, six-, or seven-membered carbocyclic or heterocyclic ring         consisting of zero to three heteroatoms, wherein the optionally         substituted group can be unsubstituted, fully substituted,         monosubstituted or substituted at a level anywhere in-between         fully substituted and monosubstituted, and R⁵ can be MS active,         but when R⁵ is MS active, R³ and R⁴ are H;     -   R⁶ is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone and can be MS active;     -   R⁷ is independently selected from —H or methyl; and     -   Y is a counterion.

Furthermore, provided herein are compounds of the structural Formula IV in a salt form:

-   -   wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—;     -   R⁷ is independently selected from —H or methyl; and     -   Y is a counterion.

In addition, provided herein are compounds of the structural Formula IV-A:

-   -   or a salt or solvate thereof, wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

Further provided are compounds of the structural Formula IV-B:

-   -   or a salt or solvate thereof, wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

Compounds of the structural Formula V are provided in a salt form as:

-   -   wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is independently selected from O═C═N—, S═CαN,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N;     -   R⁷ is independently selected from —H or methyl; and     -   Y is a counterion.

The invention further includes compounds of the structural Formula V-A:

-   -   or a salt or solvate thereof, wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N.

The invention also includes compounds of the structural Formula V-B:

-   -   or a salt or solvate thereof, wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is independently selected from O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N.

In addition provided herein are compounds of the structural Formulas VI, VII, VIII, VIII-A, VIII-B, IX, IX-A, and IX-B which are useful for rapid fluorescent labeling of glycans and subsequent analysis by means of liquid chromatography and mass spectrometry and provided immediately below. Furthermore, the Formula IV, IV-A, IV-B, V, V-A, V-B, VIII, VIII-A, VIII-B, IX, IX-A, and IX-B described herein may be fully substituted with more than one R₂ group as defined therein.

-   -   wherein     -   R¹ is independently selected from O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

-   -   wherein     -   m=0-9;     -   n=0-9;     -   R¹ is independently selected from O═C═N—, S═C═N—

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N.

-   -   wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

-   -   wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R¹ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

-   -   wherein     -   X═C or N;     -   R¹ is independently selected from O═C═N—,

-   -   R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring but not when R¹ is         O═C═N—.

-   -   wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is independently selected from O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N.

-   -   wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is independently selected from O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R¹         is S═C═N.

-   -   wherein     -   m=0-9;     -   n=0-9;     -   X═C or N;     -   R¹ is independently selected from O═C═N—, S═C═N—,

-   -   R² is independently selected from methylene, substituted         nitrogen, oxygen, carbonyl, amide, ester, sulfur, sulfoxide, or         sulfone;     -   R³ and R⁴ are independently selected from —H, alkyl, alkyl         amino, alkylsulfonic acid, alkyl phosphonic acid, wherein R³ or         R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid,         and wherein R³ and R⁴ together with the nitrogen to which they         are attached may form an optionally substituted 5- to 8-membered         saturated or partially unsaturated ring; and     -   R⁵ is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈         cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl,         alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—, and not when R₁         is S═C═N.

The following schemes I, II, III and IV can be used to practice the invention and make the compounds described herein.

Preparation of 2,5-dioxopyrrolidin-1-yl(2-((2-(diethylamino)ethyl)carbamoyl)quinolin-6-yl)carbamate

40 mg of B was dissolved in 2.5 mL of a 1:4 mixture of dimethylformamide:dichloromethane in a 10 mL flask equipped with a stir bar and purged with N₂. 1.7 mg of dimethylaminopyridine and 181 μL of dicyclohexylcarbodiimide were then added to the flask. After stirring for 10 min, 2-(diethylamino)ethylamine (57 mg) in 3 mL of dichloromethane was added to the flask. This was then stirred at room temperature for 20 hours. After this time, 3 mL of water was added to the reaction flask. The organic layer was separated and the aqueous layer was extracted with 2 mL of dichloromethane. The organic phases were combined, dried, and then evaporated to dryness to provide the crude material. This was subjected to standard organic chemistry purification techniques to provide the desired material C in >95% purity.

1.8 g of C was dissolved in a mixture of 5.3 g of trifluoracetic acid in 30 mL of dichloromethane. The reaction mixture was stirred at room temperature for 48 hours. After removal of the solvent under reduced pressure, the crude material was dissolved in 30 mL of 0.5 N HCl. This mixture was then extracted with 50 mL aliquots of ethyl acetate. The organic phases were combined, dried, and then evaporated to dryness to yield 1.2 gram of the crude product. This was subjected to standard organic chemistry purification techniques to provide the desired material D in >98% purity.

425 mg of D was added to 6 mL of dry acetonitrile in a dry 10 mL Erlenmeyer flask equipped with a stir bar, and allowed to dissolve. In a separate 25 mL flask equipped with a 10 mL dropping funnel and a stir bar, 380 mg of N,N-disuccinimidylcarbonate (DSC) was dissolved in 6 mL of dry acetonitrile, and the system was purged with N₂. The solution of D was then transferred to the dropping funnel, and added dropwise to the DSC solution over the course of 10 min. The solution was then allowed to stir for 4 hours. At this point, the solvent was removed, and the desired product E was dried at room temperature under high vacuum.

Preparation of 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate

In a first method, 2.6 g of procainamide A was added to 47 g of dry acetonitrile in a dry 100 mL Erlenmeyer flask equipped with a stir bar, and allowed to dissolve. In a separate 1 L flask equipped with a dropping funnel and a stir bar, 3.2 g of N,N-disuccinimidylcarbonate (DSC) B was dissolved in 417 g of dry acetonitrile, and the system was purged with N₂. The solution of procainamide was then transferred to the dropping funnel, and added dropwise to the DSC solution over the course of 1 hour. The solution was then allowed to stir for 4 hours. At this point, the solvent was removed, and 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate C was dried at room temperature under high vacuum.

In a second method, 8.2 g of procainamide was added to 50 mL of dry dichloromethane in a dry 100 mL Erlenmeyer flask equipped with a stir bar, and allowed to dissolve. In a separate 1 L flask equipped with a dropping funnel and a stir bar, 10.1 g of N,N-disuccinimidylcarbonate (DSC) was mixed with 400 mL of dry dichloromethane, and the system was purged with N₂. The solution of procainamide was then transferred to the dropping funnel, and added dropwise to the DSC solution over the course of 1 hour. The solution was then allowed to stir for 4 hours. At this point, the desired product was removed from the mother liquor by filtration, then dried at room temperature under high vacuum.

Methods used for Scheme I and Scheme II are applicable to Scheme III for making the compounds presented herein.

Methods used for Scheme I and Scheme II are applicable to Scheme IV for making the compounds presented herein.

Therefore, specific novel compounds useful in the rapid fluorescence tagging of molecules with enhanced MS signals include, but are not limited to, the following compounds:

N-linked and O-linked glycans are common glycans from recombinant biotherapeutic proteins, N-glycans being the more prominent. N-linked glycans are attached to asparagines via an N-acetylglucosamine (“GlcNAc”) residue in an Asn-Xxx-(Ser, Thr) motif where Xxx can be any amino acid except proline. O-linked glycans are attached to either Serine or Threnine. N-linked glycans can be removed from the glycoprotein chemically or enzymatically. Analytical methods of analyzing N-linked glycans have become considerably sophisticated. CE-, HPAEC-PAD, HILIC-LC/FLR, RPLC/MS, MALDI-MS are the most common analytical instrumentations. Liquid chromatography (“LC”) separation with fluorescence detection is widely used in the pharmaceutical industry for the characterization of enzymatically/chemically released glycan, typically tagged with a fluorescent dye at the reducing end of a glycan. Kalyan R. Anumula & Shirish T. Dhume, High Resolution and High Sensitivity Methods for Oligosaccharide Mapping and Characterization by Normal Phase High Performance Liquid Chromatography Following Derivatization with Highly Fluorescent Anthranilic Acid, 8 GLYCOBIOLOGY 685 (1998); Karina Mariňo et al., A Systematic Approach to Protein Glycosylation Analysis: A Path Through the Maze, 6 NATURE CHEMICAL BIOLOGY 713 (2010). Fluorescent measurements are sensitive and quantitative; the low detection limit is in the low femtomoles. With recent advancements in mass spectrometry instrumentation, the combination of liquid chromatography, fluorescence and MS has gained more popularity as an analytical instrument platform for routine characterization of fluorescently labeled N-linked glycans. Therefore, relative quantitation and molecular weight measurements can be done in a single analysis. Shigeo Suzuki et al., Comparison of the Sensitivities of Various Derivatives of Oligosaccharides in LC/MS with Fast Atom Bombardment and Electrospray Ionization Interfaces, 1006 ANAL. CHEM 2073 (1996). However, another challenge is that glycans do not ionize efficiently via electro-spray-ionization (“ESI”). Therefore, in general, tagging with an MS active moiety is required.

The sample preparation step can be very time consuming as it requires enzymatic digestion on the protein to release N-linked glycans followed by fluorescence tagging reaction. For example, the derivatization with a fluorescence moiety accomplished by reductive amination can require up to 4 hours. Derivatization using the aromatic amine, 2-aminobenzamide (2AB), is the most established method and requires this reductive amination. The 2AB tag improves the MS sensitivity compared to the non-labeled glycan and is fluorescently active.

Provided herein is a rapid method for fluorescent tagging N-linked glycans using novel chemical reagents. These tags are designed to enhance the analyte mass spectrometry response. This same chemical tag maybe used for amino acid and peptide labeling. The reaction mechanism may be the same for all three types of molecules, whereby the derivatization occurs at the amine moiety. Amino acid analysis, peptide mapping and glycan profiling are each an integral part of the overall biotherapeutic protein characterization. Therefore, it is advantageous to have rapid universal fluorescent derivatization methods which improve detection of the MS instrumentation.

New molecules (also referred herein to as “reagents”) specific for N-linked glycans amino acids and peptides, are provided for enhanced MS detection and rapid fluorescence tagging of glycans and other biomolecules with enhanced MS signals. Through the use of these reagents, the reaction times necessary to carry out the tagging process (or otherwise sometimes referred to herein as “labeling”) is measured in seconds, rather than minutes or hours. The described molecules are useful in a wide variety of processes that rely on glycan and amino acid/peptide analysis for essential information of a product, process, or protein. As such, the molecules described herein may be used in processes such as protein characterization, cell culture monitoring, synthetic peptide manufacturing, and food analysis.

The reagents provided herein can consist of three functional components a) a tertiary amino group or other MS active atom, b) a highly fluorescent moiety, and c) a functional group that rapidly reacts with amines (such as an isocyanate, or succidimidylcarbamate). Other reagents can consist of two functional components a) a tertiary amino group or other MS active atom, and b) a functional group that rapidly reacts with amines (such as an isocyanate, or succidimidylcarbamate). The components serve the following purposes: (1) the amino group or MS active group gives good MS signal; (2) the fluorescent moiety provides a good fluorescence signal; and (3) the reactive functional group gives rapid tagging of desired biomolecules.

The enhanced MS signal observed upon utilization of a 2AB tagging reagent (slow reaction-hours), for example, is a function of the amino or amine group present in the system following tagging. Currently available rapid tagging agents contain no amino functionality following tagging of the desired biomolecule. Rather, these compounds have functionalities which do not provide the same electron density for mass spectrometry applications (e.g., urea or carbamate) and tie up the electron density resulting in low MS signal.

Biomolecules are organic compounds that are involved in the maintenance and metabolic processes of living organisms. Many disease conditions are due to impaired amino acid metabolism (e.g. phenylketonuria). As noted above, a biomolecule can also be a therapeutic agent such as peptide based pharmaceuticals that have been used to treat the disease. Glycans, amino acids, peptides and proteins are closely monitored during protein drug development and production. Many biomolecules can be detected by tagging them with a fluorescent label. The resulting conjugate or complex will show fluorescence, thereby facilitating their detection. There is recent movement in the industry towards using MS for detection and quantitation of biomolecules. Fluorescent detection is still widely used for its sensitivity and quantitative analysis. Therefore, the combination of liquid chromatography, mass spectrometry and fluorescent detection is an analytical platform that can be used for a comprehensive protein analysis. Notwithstanding, there is no single technique that is capable of providing a complete structural analysis of N-linked glycans.

Mammalian and plant glycoproteins are biomolecules that commonly contain any one or more three types of constituent glycans, oligo- and polysaccharides. Glycans are important for protein folding and any alteration thereof may eliminate or alter activity. Often an immune response is triggered by an unrecognized glycan. Of the three types of glycans, analytical methods of analyzing N-linked glycans have become considerably sophisticated.

Structurally, N-linked glycans are attached to asparagines via an N-acetylglucosamine (GlcNAc) residue in an Asn-Xxx-(Ser, Thr) motif where Xxx can be any amino acid except proline. The N-glycan can be removed from the glycoprotein with hydrazine whether manually or with the aid of automated hydrazinolysis equipment. The reagent cleaves peptide bonds between the N-linked glycan and asparagines to produce the glycan biomolecule. Several enzymes are available for releasing N-glycans. N-glycosidase F (PNGase-F), a commonly used enzyme, cleaves the intact glycan as the glycosylamine leaving aspartic acid in place of the asparagine at the N-linked site of the protein. Harvey, D. J., Identification of Protein-Bound Carbohydrates by Mass Spectrometry, 1 PROTEOMICS 311 at 311-312, 317 (2001), incorporated herein by reference.

Detection of Molecules by MS and Fluorescence

Most amino acids and/or glycans are not readily detectable due to the absence of a strong chromophore or fluorophore or MS active moiety. The absorbance and fluorescence response are quite weak. A commonly used tactic to maximize the sensitivity of an assay is to convert the compound of interest into a derivative that exhibits a better response for the particular detection method being utilized. The selection of a derivatizing agent is a critical choice in the development of an analytical procedure. The derivatizing agent affects the ultimate sensitivity and accuracy of the analysis by maximizing the sensitivity, yield and stability of the derivatized molecules.

Basically, the following determinations must be performed separately: (1) the glycosylated sites; (2) the glycosylated site occupancy; (3) the structure and amount of each glycan at each site; and (4) the number of glycoforms. Id. at 312, incorporated herein by reference. In most situations, MS can provide the answers to each of these steps. Hence the need for enhanced MS signals. Because of the branched nature of the glycan, however, structural determination of the glycan is complicated. Here, the monosaccharide unit, the anomericity and ring size of each monosaccharide, the monosaccharide sequence and ring conformation together with identification of other groups must be determined. With the exception of ring conformation, MS can be used directly or indirectly to make these determinations using MALDI and/or ESI as the preferred MS technique. Id. at 313-316, incorporated herein by reference.

Currently, for N-glycans, derivatives are most commonly added to the reducing terminus of the glycan by reductive amination reaction with an aromatic amine. Id. at 318-319. Reducing-Terminal Derivatization, incorporated herein by reference. Reductive amination, while producing an MS active compound, is a very slow process and can take four (4) hours to tag the reagent to the compound. Reducing-terminal derivatives may also be prepared by reactions other than reductive amination. Id. at 319, incorporated herein by reference.

Most glycans are not readily detectable due to the absence of a strong chromophore or fluorophore. Free glycans released from glycoproteins enzymatically or chemically can be analyzed directly via MALDI MS or ESI/MS/MS directly without any chemical tagging. Ying Qing Yu et al., A Rapid Sample Preparation Method for Mass Spectrometric Characterization of N-linked Glycans, 19 RAPID COMM. MASS SPECTROMETRY 2331 (2005). This label-free approach is suitable for qualitative analysis for glycans. However, this approach is not as well suited for relative quantitation due to the fact that glycans from a single protein sample can be very heterogeneous in that the ionization efficiency is not the same among them. Therefore, a single analysis platform that can perform both quantitive and qualitative analysis is desirable. Since a fluorescent detector only detects the dye itself, the fluorescent response from various glycans can be used for relative quantitation. The selection of a derivatizing agent is a critical choice in the development of an analytical procedure. For N-glycans, derivatives are often added to the reducing terminus of the glycan by reductive amination reaction with an aromatic amine. Reductive amination, while producing an MS active compound, is a very slow process and can take up to four hours to complete. There are many aromatic amine compounds that are used for reductive amination for glycans, most of them giving a low to moderate MS response. Recently, it was reported that procainamide can be used to enhance glycan MS response. Song Klapoetke et al., The Evaluation of a Novel Approach for the Profiling and Identification of N-linked Glycans With a Procainamide Tag by HPLC With Fluorescent and Mass Spectrometric Detection, 53 J. PHARMACEUTICAL AND BIOMEDICAL. ANAL. 315 (2010). A significant increase of glycan ionization was observed when compared with 2AB-labeled glycans. Id. However, procainamide labeling procedure is similar to other commonly used reductive amination reagents, and therefore, it still takes a half day for the labeling step.

As such, fluorogenic derivatization prior to an HPLC analysis of amino acids currently serves as an efficient tool in the analysis of these systems. For example, phanquinones and benzooxadiazoles are nitrogen containing fluorophores that can be used as pre-column derivatization agents. These compounds are devoid of intrinsic fluorescence. However, on conjugation with amino acids, they form the corresponding fluorescent conjugates.

Uses of the Reagent Compounds Presented Herein

The present compounds are particularly useful for derivatizing glycans and also amino acids and proteins because they react quickly with the molecules and form a stable, highly fluorescent MS derivative. The general methodology for an analysis of a glycan or amino acid using the compounds of the subject invention consists of three closely related processes: (1) formation of derivatives in the sample; (2) separation of the derivatives; and (3) detection of the separated derivatives. The first step is generally performed by reacting a mixture with one of the present reagents to yield a distinct compound. These derivatives provide a fluorescent signal which can then be detected in the detection stage of the analysis.

The separation step is based upon the differences in the chemical structure of the derivatives. The derivatized amino acids differ from each other in the same way that the chemical structures of the precursor amino acids differ. The derivatives must be separated so that the detector signal can be correctly related to the concentration of each derivative. The derivatized amino acids can be separated and detected by chromatography, e.g., by high performance liquid chromatography (HPLC) or capillary zone electrophoresis (CZE). HPLC is particularly useful for this purpose. These technologies are well suited for this purpose because they are selective and can be used with very small samples. It is also possible to carry out the separation step by separating the amino acids prior to their derivatization.

The detection step is generally carried out using either an absorbance or fluorescence detector. As each derivative is eluted from the chromatographic column after separation, its presence and quantity is detected by a mass spectrometer and/or by the aborbance or emission of light. The sensitivity of the assay depends upon the strength of the signal produced.

In the case of peptide analysis, reverse phase HPLC can be also used to analyze the peptide digests. In a given peptide digest there may be from 20 to 150 different peptides, each of which must be resolved and quantified. In many instances, the available sample is very small. For example, the analyst may be determining the structure of a protein that is isolated from an organism or one that has been synthesized by recombinant DNA technologies. Typically, nanomole quantities of a protein digest are studied. Due to the scarcity and cost of many proteins, it is very desirable to use as small a sample as possible.

As described in Example I below, we tested the labeling of N-linked glycans with 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate. Here, N-linked glycans were released from a glycoprotein (Herceptin) using PNGase F prior to labeling with 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate. The 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate was solubilized in water free acetonitrile to a final concentration of 45 μg/μl. 100 μl of 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate solution was added to the released glycan sample and left at room temperature for 5 minutes, during which time the labeling reaction finished. The labeled sample was lyophilized using a speed vac. The lyophilized sample was reconstituted in 60% acetonitrile/water solution prior to chromatographic separation using HILIC LC method. As shown in FIGS. 1 and 2, the samples were analyzed using fluorescence and MS detection.

Additional Uses for the Molecules Presented Herein

Absorbance detection is generally used in protein mapping work. Two different detection processes which are often used for this purpose are: a) detection at 210-215 nm using a single wavelength detector; and b) broadband spectral detection using a photodiode array (PDA) detector. In the first method, all peptides absorb at that wavelength, thus the user can ensure that all peptides eluted from the column are detected. One difficulty with this technique is that a wide variety of compounds absorb in this region of the spectrum, and extreme care must be taken to ensure that all reagents, eluents, glassware, etc. are scrupulously clean to ensure that the observed signal is solely from the peptides. In the second method, the PDA detector collects the spectra of the eluent at specific time intervals (e.g. a spectrum between 200 and 350 nm is collected every second). This provides more information than a single wavelength and thus can assist in distinguishing between peptides which may elute with similar retention times.

Peptide mapping often involves the qualitative and quantitative analysis of trace levels of peptides in the digested protein. The identification and quantitation of peptides in complex mixtures in the present method is effected by a three stage process: a) tagging the peptides of interest with the heterocyclic aromatic carbamates or other reactive groups, which exhibit a stronger absorbance or fluorescence signal than the original compound; b) separating the derivatized samples; and c) detecting the derivatized peptides by absorbance or fluorescence techniques. The separation conditions for a derivative are frequently drastically different from the separation of the starting compounds. Likewise, the efficiency of a separation has a serious impact on the detection process. Use of the present heterocyclic aromatic carbamates and/or similar reactive groups provides a mapping method adaptable for use with nanogram quantities of protein. Further, the methods described herein provide a means for enhancing the sensitivity of known methodologies for detecting peptides in biological samples, such as tissue, urine, blood and saliva.

Derivatization Agent Selection

There are several criteria important for the utility of a derivatization method. The analytical procedure must provide accurate quantitation of each component present in a complex mixture. To accomplish this, it is necessary to resolve the components of interest, not only from each other, but from components generated by the derivatization procedure. Quantitative conversion of all underivatized glycans and amino acids, including secondary amino acids, to single products is highly desirable, and facilitates good quantitation.

Detection selectivity is another advantageous feature for amino acid derivatives. Underivatized amino acids all absorb weakly in the low UV (200-220 nm) range, but detection at such wavelengths is subject to interference by many compounds present in sample mixtures or chromatographic mobile phases. Derivatization with reagents absorbing at approximately 254 nm provides a measure of selectivity, but any aromatic organic compounds, frequently present in biological samples, can interfere at this wavelength. Reagents that enable detection via fluorescence, electrochemical response or visible-range absorbance would be desirable for superior detection selectivity.

Finally, it is necessary for derivatives to be sufficiently stable to allow separation and detection without significant degradation. Highly stable derivatives are also favorable as they allow a sample to be reanalyzed, if so desired, without assaying another sample.

In the past, a number of derivatization procedures have been developed to permit the assay of amino acids by high performance liquid chromatographic and electrophoretic separations. Five such procedures commonly utilized for this purpose include:

(1) The o-phthalaldehyde (OPA)/mercaptan method. The OPA procedure can detect amino acids with a typical detectable level in the order of about 100 femtomole (fmol). The formation of the derivatives is rapid. A significant difficulty with this method is the adduct is fairly unstable, and must be prepared very shortly before the detection step. An additional problem is that this reagent will not form a derivative with secondary amino acids.

(2) The 9-fluorenylmethylchloroformate (FMOC method). The FMOC procedure provides for stable derivatives, with a minimum detectable level in the order of a few hundred fmol. There are a number of disadvantages with the FMOC procedure. Free tryptophan and cystine cannot be quantitated easily. The derivatizing reagent must be removed from the reaction mixture by an extraction step because it is itself fluorescent. The reagent has also been reported to form multiple derivatives with histidine. The reagent is also hazardous to work with, because it is corrosive and is a lachrymator.

(3) The phenylisothiocyanate method (PITC). The PITC procedure yields stable derivatives which are formed rapidly. It can be used for both primary and secondary amino acids, as well as cystine. The method uses absorbance as the detection procedure, and can provide a minimum detection limit of 1 pmol. However, the derivatives are not fluorescent and detection must be performed at 254 nm, which does not allow for good detection selectivity.

(4) The dansyl chloride method. The dansyl chloride method provides stable derivatives with a minimum detectability in the order of about 1.5 pmol. It is able to detect secondary amines and cysteine, but it results in multiple derivatives.

(5) Fluorescent succinimidocarbamates have been used as derivatizing agents for amines, amino acids, peptides, phosphates and other classes of compounds. When the succinimidocarbamate reagent is used to tag a compound with a fluorescent group, a detection limit of about 1 pmol can be achieved. These reagents are used in conjunction with modern separation techniques such as high performance liquid chromatography, thin layer chromatography or capillary electrophoresis. Nimura et al., 58 ANAL. CHEM. 2372 (1986). Succinimidyl activated carbamates have been prepared by reacting carbocyclic aromatic amines with di-(N-succinimidyl) carbonate. Takeda et al., 24 TETRAHEDRON LETT., 4569 (1983).

Current derivatization chemistry for HPLC analysis of a broad range of samples includes Waters' AccQTag Amino Acid Analysis System. Waters AccQTag method is a precolumn derivatization technique for peptide and protein hydrolysate amino acids. The AccQTag methodology is based on a derivatizing reagent developed specifically for amino acid analysis. Waters AccQFluor reagent (6-aminoquinolyl-N-hydrozysuccinimidyl carbamate, or ACQ) is an N-hydroxysuccinimide-activated heterocyclic carbamate, a known class of amine-derivatizing compounds. See, EP0533200 B1.

This reagent converts both primary and secondary amino acids to stable, fluorescent derivatives and hydrolyzes to yield 6-aminoquinoline, a non-interfering byproduct. The AccQFluor reagent reacts rapidly with primary and secondary amino acids to yield highly stable ureas that fluoresce strongly at 395 nm. The resulting derivatives are stable at room temperature for up to one (1) week.

The key to the rapid and simple Waters AccQTag Amino Acid Analysis method is the derivatizing reagent and a simple, pre-column derivatization protocol. Waters AccQFluor Reagent is a highly reactive compound, 6-aminoquinolyl-N-hydroxysuccinimidyl carbarmate (AQC), which forms stable derivatives with primary and secondary amino acids in a matter of seconds. The derivatives are easily separated by reversed phase HPLC using Waters AccQTag Amino Acid Analysis System in less than 35 minutes.

Excess reagent is consumed during the reaction to form aminoquinoline (AMQ). AMQ has significantly different spectral properties than any of the derivatized amino acids. This allows for the programming of a detector wavelength that maximizes the spectral emission response of the derivatives while minimizing the response of the AMQ. The derivatization protocol—adding reagent to and heating a properly buffered sample—is simple and straight forward. The amino acid derivatives can be injected directly without further sample preparation. Common buffer salts and detergents have little effect on reaction yield or on the reproducibility of results.

Another example of derivatization chemistry for HPLC analysis of a broad range of samples is the InstantAB™ kit, a labeling kit used to produce tagged N-linked glycans and currently manufactured by Prozyme, for example, the Glyko® InstantAB™ kit (available from Prozyme, Inc., Hayward, Calif.) a sample preparation kit used to produce tagged N-linked glycans of the formula

InstantAB™, a compound of the formula

provides rapid tagging and strong fluorescence but produces a weak MS signal. In fact, the MS signal of this molecule is significantly reduced when compared to the standard 2-AB reagent.

Presented herein are molecules that contain a tertiary amine, a fluorescent moiety and a reactive functional group. Certain other molecules may simply contain the tertiary amine and reactive functional groups. All molecules, however, undergo rapid functionalization. Through the use of a tertiary amine, all of the molecules are MS active (active in mass spectrometry), while others may also be fluorescent.

Below is a new molecule and reagent useful in mass spectrometry application and are fluorescent:

2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate

As noted above, the current state of the art utilizes tagging molecules that either 1) react very slowly and give good MS/fluorescence signals or 2) react quickly and have good fluorescence signal, but have poor MS signal. The lack of MS signal in the currently rapid reacting molecules is believed to arise from the lack of an electron rich amine—any nitrogen present loses electron density as part of urea or carbamate functionality. Herein, we provide the addition of an amino group or other MS active atom to the fluorescent moiety of the rapidly reacting systems, or alternatively, adding a reactive functional group to the slow reacting (high fluorescence and MS signal) system in order to decrease reactivity time (from hours to seconds).

Other fluorescent moieties may be useful in connection with the molecules described herein include coumarins, an important class of oxygen-containing fluorophores commonly used for labeling amino acids. Coumarins are a variety of substituted coumarins having a base molecular structure as follows:

A specific example is coumarin 7 (3-(2′-Benzimidazolyl)-7-N,N-diethylaminocoumarin) where the red R group (H on the parent coumarin) can be an isocyanate, an O-succinimidylcarbamate, or other amine reactive group.

Another example is a functionalized coumarin 334 (2,3,5,6-1H,4H-Tetrahydro-9-acetylquinolizino-[9,9a,1-gh]-coumarin) utilizing the carbonyl group as a functional handle to attach the reactive moiety.

Also, the following Nile Red derivative is functionalized through the phenol group to give an amine reactive group.

Nile Red Derivative Other examples of coumarins useful as a fluorescent moiety include:

-   Coumarin 4 (7-Hydroxy-4-methylcoumarin); -   Coumarin 120 (7-Amino-4-methylcoumarin); -   Coumarin 2 (7-Amino-4-methylcoumarin); -   Coumarin 466 (7-Diethylaminocoumarin); -   Coumarin 47 (7-Diethylamino-4-methylcoumarin); -   Coumarin 102     (2,3,5,6-1H,4H-Tetrahydro-8-methylquinolizino-[9,9a,1-gh]-coumarin); -   Coumarin 152A (7-Diethylamino-4-trifluormethytcoumarin); -   Coumarin 152 (7-Dimethylamino-4-trifluormethylcoumarin); -   Coumarin 151 (7-Amino-4-trifluormethylcoumarin); -   Coumarin 6H     (2,3,5,6-1H,4H-Tetrahydroquinolizino-[9,9a,1H-gh]coumarin); -   Coumarin 307 (7-Ethylamino-6-methyl-4-trifluormethylcoumarin); -   Coumarin 500 (7-Ethylamino-4-trifluormethylcoumarin); -   Coumarin 314     (2,3,5,6-1H,4H-Tetrahydro-9-carboethoxyquinolizino-[9,9a,1-gh]coumarin); -   Coumarin 510     (2,3,5,6-1J-4H-Tetrahydro-9-(3-pyridyl)-quinolizino-[9,9a,1-gh]coumarin); -   Coumarin 30     (3-2′-N-Methylbenzimidazolyl)-7-N,N-diethylaminocoumarin); -   Coumarin 552 (N-Methyl-4-trifluormethylpiperidino-[3,2-g]-coumarin); -   Coumarin 6 (3-(2′-Benzothiazolyl)-7-diethylaminocoumarin); and -   Coumarin 153     (2,3,5,6-1H,4H-Tetrahydro-8-trifluormethylquinolizino-9,9a,1-gh]coumarin).

Rhodamines may also be useful as fluorescent moieties. Rhodamine dyes based upon the fluorone molecule having a base molecule structure as:

and include the following examples:

-   Rhodamine 110 (o-(6-Amino-3-imino-3H-xanthen-9-yl)-benzoic acid); -   Rhodamine 19 (Benzoic     Acid,2-[6-(ethylamino)-3-(ethylimino)-2,7-dimethyl-3H-xanthen-9-yl],perchlorate); -   Rhodamine 6G (Benzoic     Acid,2-[6-(ethylamino)-3-(ethylimino)-2,7-dimethyl-3H-xanthen-9-yl]-ethylester,monohydrochloride); -   Rhodamine B     (2-[6-(Diethylamino)-3-(diethylimino)-3H-xanthen-9-yl]benzoic acid); -   Rhodamine 101     (8-(2-Carboxyphenyl)-2,3,5,6,11,12,14,15-octahydro-1H,4H,10H,13H-diquinolizino[9,9a,1-bc:9′,9a′,1-hi]xanthylium     Perchlorate).

Fluoresceins may also be useful as fluorescent moieties as shown below:

Examples include of fluoresceins include:

-   Uranin (Disodium Fluorescein); and -   Fluorescein 27 (2,7-Dichlorofluorescein)

In short, the MS active, fluorescent rapid tagging glycan molecules described herein can utilize different other fluorescent moieties in place of the benzamide structure of Formula I and II.

For example, other fluorescent moieties that may be used together with a functional group and the MS active atom to produce an MS active, rapid tagging molecule include a biphenyl-phenyl, naphthyl-substituted oxadiazol moiety as shown immediately below.

Examples include:

-   Butyl-PBD (2-(4-Biphenylyl)-5-(4-t-butylphenyl)-1,3,4-oxadiazol); -   PBD (2-(4-Biphenylyl)-S-phenyl-1,3,4-oxadiazol); and -   BBD (2,5-Bis-(4-biphenylyl)-1,3,4-oxadiazol).

Similarly, phenyl-substituted oxazols (shown immediately below) and furans (not shown) may be useful as the fluorescent moiety.

Examples of such moieties include:

-   PPO (2,5-Diphenyloxazol); -   α-NPO (2-(1-Naphthyl)-5-phenyloxazol); -   BBO (2,5-Bis-(4-biphenylyl)-oxazol); and -   POPOP (1,4-Di[2-(5-phenyloxazolyl)]benzene).

In addition, other possible fluorescent moieties are shown below:

Ter and Quaterphenyls (shown above), where the examples include:

-   TMQ (3,3′,2″,3″-Tetramethyl-p-quaterphenyl); -   BMQ (2,2′″-Dimethyl-p-quaterphenyl); -   DMQ (2-Methyl-5-t-butyl-p-quaterphenyl); -   PQP (p-Quaterphenyl); -   Polyphenyl 1 (p-Quaterphenyl-4-4′″-disulfonicacid Disodium salt); -   Polyphenyl 2 (p-Quaterphenyl-4-4′″-disulfonicacid Dipotassium salt; -   BiBuQ (4,4′″-Bis-(2-butyloctyloxy)-p-quaterphenyl); -   BM-Terphenyl (2,2″-Dimethyl-p-terphenyl); and -   PTP (p-Terphenyl).

Azaquinolone and carbostyryls (shown above), where the examples include:

-   Carbostyryl 7 (7-Amino-4-methylcarbostyryl); -   Carbostyryl 3 (7-Dimethylamino-4-methylquinolin-2); and -   Quinolon 390 (7-Dimethylamino-1-methyl-4-methoxy-8-azaquinolone-2).

Benzoxazoles and benofurans and benzothiazoles (shown above), where examples include:

-   DASBTI (2-(p-Dimethylaminostyryl)-benzothiazolylethyl Iodide); -   Coumarin 6 (3-(2′-Benzothiazolyl)-7-dimethylaminocoumarin); -   Styryl 9M     (2-(6-(4-Dimethylaminophenyl)-2,4-neopentylene-1,3,5-hexatrienyl)-3-methyl-benzothiazolium     Perchlorate); -   Styryl 15     (2-(6-(9-(2,3,6,7-Tetrahydro-1H,5H-benzo(i,j)-chinolizinium))-2,4-neopentylene-1,3,5-hexatrienyl)-3-methylbenzothiazolium     Perchlorate); -   Styryl 14     (2-(8-(4-p-Dimethylaminophenyl)-2,4-neopentylene-1,3,5,7-octatetraenyl)-3-methylbenzothiazolium     Perchlorate), -   Styryl 20     (2-(8-(9-(2,3,6,7-Tetrahydro-1H,5H-benzo(i,j)-chinolizinium))-2,4-neopentylene-1,3,5,7-octatraenyl)-3-methylbenzothiazolium     Perchlorate); -   Furan 1 (Benzofuran,2,2′-[1,1′-biphenyl]-4,4′-diyl-bis-tetrasulfonic     acid (tetrasodium salt)); and -   PBBO (2-(4-Biphenylyl)-6-phenylbenzoxazol-1,3).

Likewise, substituted stilbenes (phenyl, diphenyl, napthyl, etc. . . . ) shown immediately below might be used as the fluorescent moiety in an MS, rapid tagging fluorescent molecules. Substituted stilbenes have the base structure as follows:

Examples of substituted stilbenes (shown above) include:

-   DPS (4,4′-Diphenylstilbene); -   Stilbene 1 ([1,1′-Biphenyl]-4-sulfonic acid,     4′,4′″-1,2-ethene-diylbis-, dipotassium salt); and -   Stilbene 3     (2,2′-([(1,1′-Biphenyl]-4,4′-diyldi-2,1-ethenediyl)-bis-benzenesulfonic     acid disodium salt).

Likewise, as noted above, fluoro/rhodamine moieties (shown below) may be used as the fluorescent moiety:

An example is Fluorol 7GA (2-Butyl-6-(butylamino)-1H-benz[de]isoquinoline-1,3(2H)-dione).

Sulforhodamine dyes are also useful in connection with the molecules presented herein. Sulforhodamine dyes have a base molecule structure as follows:

Here, examples include:

-   Sulforhodamine B (Ethanaminium,     N-[(6-diethylamino)-9-(2,4-disulfophenyl)-3H-xanthen-3-ylidene]-N-ethylhydroxid,     inner salt, sodium salt); and -   Sulforhodamine 101     (8-(2,4-Disulfophenyl)-2,3,5,6,11,12,14,15-octahydro-1H,4H,10H,13H-diquinolizino[9,9a,1-bc:9′,9a′,1-hi]xanthenes).

Pyrromethenes could also serve as the fluorescent moiety in the MS active rapid tagging molecules having the general structure below:

Examples of pyrromethenes include:

-   Pyrromethene 546 (1,3,5,7,8-pentamethylpyrromethenedifluoroborate     complex); -   Pyrromethene 556     (Disodium-1,3,5,7,8-pentamethylpyrromethene-2,6-disulfonate-difluoroborate     complex); -   Pyrromethene 567     (2,6-Diethyl-1,3,5,7,8-pentamethylpyrromethenedifluoroborate     complex); -   Pyrromethene 580     (2,6-Di-n-butyl-1,3,5,7,8-pentamethylpyrromethenedifluoroborate     complex); -   Pyrromethene 597     (2,6-Di-t-butyl-1,3,5,7,8-pentamethylpyrromethenedifluoroborate     complex); and -   Pyrromethene 650     (8-Cyano-1,2,3,5,6,7-hexamethylpyrromethenedifluoroborate complex).

Furthermore, phenoxazonium (shown immediately below) and phenoxazine may also be useful fluorescent moieties:

Examples of these fluorescent moieties include:

-   Cresyl Violet (5,9-Diaminobenzo[a]phenoxazonium Perchlorate); -   Nile Blue (S-Amino-9-diethyliminobenzo[a]phenoxazonium Perchlorate); -   Oxazine 170     (9-Ethylamine-5-ethylimino-10-methyl-5H-benzo(a)phenoxazonium     Perchlorate), and -   Oxazine 1 (3-Diethylamino-7-diethyliminophenoxazonium Perchlorate).

Pyrene-derivatives compose another class of fluorescent moieties that may be useful in connection with the molecules presented herein. Pyrene dyes are based upon the following basic structure:

Examples of pyrene-derivatives include:

-   N-(1-pyrene)maleimide; and -   Pyranine (trisodium 8-hydroxypyrene-1,3,6-trisulfonate) The     fluorescent molecules and other fluorophores can be used.

Ideally, fluorescent molecules are those molecules that produce a fluorescent signal in the range of about 300 to 700 nanometers. Examples include rhodamines and coumarins.

Reactive functional groups can include succidimidylcarbamate and isocynate.

The present molecules can have heterocyclic aromatic groups that exhibit a higher fluorescence quantum yield that that of carbocyclic aromatics used as tags. Nimura et al Anal. Chem. 58, 2372 (1986). This increase in the fluorescence quantum yield of the tag provides an increase in the sensitivity of the tagged amine. For some of the heterocyclic molecules, the emission maximum of an amine compound derivatized with the reactive group is at a significantly different wavelength than the emission maximum of the free heterocyclic amine. The wavelength shift has very significant implications for fluorescence detection of tagged amines. Furthermore, since the observed fluorescence is predominantly from the derivative, background noise is eliminated or reduced and a more sensitive assay obtained.

Sample Preparation

The molecules provided herein do not provide a work-around for proper sample preparation. To obtain high quality mass spectra, the condition of the sample is of critical importance. Compounds other than the analyte will generally have an adverse effect on ion yield and must be removed. Indeed, while small amounts of sodium are essential for ionization by MALDI, carbohydrates are particularly susceptible to the effects of salts. Moreover, many carbohydrates occur as mixtures. Therefore it is important to ensure that isolation and purification techniques do not cause fractionation of the sample with a loss of quantitative information. Exemplary is sialic acids which often are lost from glycoproteins when pH is too low or sample temperature too high.

Example I Tagging of N-Linked Glycans Released from Herceptin

N-linked glycans were released from 0.8 μg of Herceptin using standard PNGase F protocols prior to labeling with 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate. 2,5-dioxopyrrolidin-1-yl (4-((2-(diethylamino)ethyl)carbamoyl)phenyl)carbamate was solubilized in dry (water free) acetonitrile to a final concentration of 45 μg/μl. 10 μl of this solution was then added to the to the released glycan sample. This mixture was left at room temperature for 5 minutes. The labeled sample was then lyophilized using a speed vac and reconstituted in 60% acetonitrile/water solution prior to chromatographic separation using a HILIC LC method and analysis by fluorescence and MS detection, as shown in FIGS. 1 and 2. 

We claim:
 1. A compound of the structural Formula IV-A:

or a salt or solvate thereof, wherein X═C or N; R¹ is independently selected from O═C═N—,

R² is independently selected from —H, —C₁-C₈ alkyl, —C1-C₈ cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and R³ and R⁴ are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, except that in the case where R¹ is O═C═N—, the requirement that either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid is removed.
 2. A compound of claim 1, wherein the structural formula is:

or a salt or solvate thereof, and X═C or N; R¹ is independently selected from O═C═N—,

R² is independently selected from —H, —C¹-C⁸ alkyl, —C¹-C⁸ cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and R³ and R⁴ are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein either R3 or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, except that in the case where R¹ is O═C═N—, the requirement that either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid is removed.
 3. A compound of the structural Formula IV-B:

or a salt or solvate thereof, wherein X═C or N; R¹ is independently selected from O═C═N—,

R² is independently selected from —H, —C₁-C₈ alkyl, —C1-C₈ cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and R³ and R⁴ are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, except in the case where R¹ is O═C═N—, the requirement that either R3 or R4 is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid is removed.
 4. A compound of the structural Formula VIII-A:

wherein X═C or N; R¹ is independently selected from O═C═N—,

R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈ cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and R³ and R⁴ are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, except that in the case where R¹ is O═C═N—, the requirement that either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid is removed.
 5. A compound of claim 4, wherein the structural formula is:

and X═C or N; R¹ is independently selected from O═C═N—,

R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈ cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and R³ and R⁴ are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, except that in the case where R¹ is O═C═N—, the requirement that either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid is removed.
 6. A compound of the structural Formula VIII-B:

wherein X═C or N; R¹ is independently selected from O═C═N—,

R² is independently selected from —H, —C₁-C₈ alkyl, —C₁-C₈ cycloalkyl, halo, dialkylamino, CH₂-dialkylamino, aminocarbonyl, alkoxycarbonyl, or alkoxy, but not Cl or O═C═N—; and R³ and R⁴ are independently selected from —H, alkyl, alkyl amino, alkylsulfonic acid, alkyl phosphonic acid, wherein either R³ or R⁴ is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid, except in the case where R¹ is O═C═N—, the requirement that either R3 or R4 is alkylamino, alkyl phosphonic acid, or alkylsulfonic acid is removed.
 7. A compound of the formula: 